Reagent to add	Stock concentration	Volume to add	Final concentration
IMDM		725 ml
Ham’s F12		242.5 ml
N2		5 ml
B27		10 ml
7.5% BSA	7.5%	7.5 ml
Penicillin–streptomycin	100×	10 ml	1×
To make complete SFD medium, add the following to SFD
Reagent to add	Stock concentration	Volume to add per ml	Final concentration
GlutaMAX	100×	10 μl	1×
Ascorbic acid	50 mg/ml	1 μl	50 μg/ml
MTG	11.5M	0.039 μl	0.45 mM