| Complete SFD medium supplemented with | Stock concentration | Volume to add per ml | Final concentration |
|---|---|---|---|
| CHIR99021 | 3 mM | 1 μl | 3 μM |
| FGF10 | 10 μg/ml | 1 μl | 10 ng/ml |
| FGF7 (KGF) | 10 μg/ml | 1 μl | 10 ng/ml |
| BMP4 | 10 μg/ml | 1 μl | 10 ng/ml |
| All-trans RA | 0.5 mM | 0.1 μl | 50 nM |
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