Table 1.

Summary of inhibition values in binding and hedgehog functional assays for confirmed hits from chemical library screen of ShhN/heparin binding.

Cpd No.	Sample ID	ShhN/heparin Avg. IC50 (μM) a	Inhibition of ShhN-induced AP IC50 (μM)	Inhibition of SAG-induced AP	Inhibition of ShhN-induced GLI1 and Ptch1 mRNA	Inhibition of SAG-induced GLI1 and Ptchl mRNA
1	ASN03067791	38	1.6	Yes
4	BAS	53	42	No	Yes	No
6	BAS	49	No inhibition
8	BAS	48	No inhibition
10	BAS	57	23	Yes
11	BAS	46	No inhibition
20	BAS	55	No inhibition
21	BAS	45	25	Weak	Yes	Yes
24	BAS	33	26	No	Yes	No
26	BAS	44	21	Yes
	Robotnikinin	No inhibition	6.4	No	Yes	No

^aFor IC₅₀ determinations, serial dilutions of compounds were tested starting at a high concentration of 80 μM. Average IC₅₀ values from cherry-picked master plates and repurchased compounds.