FIG. 10.

SIV V1-V2 variants present in dilution series B (Fig. 9) of plasma from donor monkey 21743 at 2 weeks p.i. Lanes 1 to 3, dilutions of plasma from undiluted to 10⁻² (same as lanes 5 to 7 in Fig. 9). The V1-V2 sequence amplified from the 10⁻² dilution (∗; lanes 3 and 9) was considered to be one of the three most common envelope variants in the plasma of 21743 (see text and Fig. 9). Lanes 4 to 8 show plasma variants at week 1 p.i. from the two monkeys inoculated in serial vaginal passage 1 (Fig. 3), separately and mixed together (lanes 4 to 6) and mixed with one of the most common V1-V2 variants (lane 3) in the plasma of the donor animal (lanes 7 and 8). Monkeys 30446 and 30469 were infected with the same predominant variant (lane 6), and this variant was the same as one of the most common variants in the plasma of donor animal 21743 (lanes 7 and 8), as indicated by the formation of homoduplexes when variants from two different animals were mixed.