TABLE 2.
Replication of viruses adapted to growth in lectin-selected cellsa
| Cell line | Titer (TCID50)
|
|||
|---|---|---|---|---|
| AM2AL3 | AL3(MaKS)-13 | K4 | K4(MaKS)-13 | |
| MDCK | 1.8 × 109 | 5.6 × 104 | 5.6 × 104 | 5.6 × 104 |
| MaKS | 5.6 × 106 | 5.6 × 104 | 1.8 × 103 | 1.8 × 105 |
| Ratio, MDCK titer/MaKS titer | 321 | 1 | 31 | 0.3 |
a
The susceptibility of each cell line was determined by infecting cells with AM2AL3 (grown in eggs), K4 (grown in MDCK cells), AL3(MaKS)-13 (grown in MaKs cells), or K4(MaKS)-13 (grown in MaKs cells) stock virus and determining the dose required to infect 50% of tissue culture cells (TCID50). Note that both viruses adapted in MaKS cells grow in these cells as well as [AL3(MaKS)-13] or better than [K4(MaKS)-13] in MDCK cells, while the original viruses grow better in MDCK cells.