Fig. 4.

Dietary nitrate promotes the Sialin expression in colon epithelial cells of aging mice. a Immunofluorescence staining of MUC2 (green), Sialin (red) and DAPI (blue) in the colonic tissues in the group of young, aged and nitrate. Scale bar: 100 μm. b Quantification of the relative area of MUC2 and Sialin, n = 5 in each group. c Western blot analysis of Sialin expression in colon tissues of young, aged and nitrate, n = 3 per group. ***Indicates significance at P < 0.001, **P < 0.01. Data were shown as mean ± SD