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Content of free FA in iCCA stem-like compartment.
Relative metabolite abundance of intracellular free citric, stearic, oleic, and palmitic acid in CCLP1 or HUCCT1 cell lines, grown in MON or as SPH, identified by LC-MS analysis. Peak areas obtained were normalised for protein content for each sample. Mean ± SEM (n = 9, ∗∗p ≤0.01, ∗∗∗p ≤0.001; Mann–Whitney U test). FA, fatty acids; iCCA, intrahepatic cholangiocarcinoma; MON, monolayer; SPH, sphere cultures.
