Figure 4.

Predicted performance of the gsTLS for H&E-based TLSs status in the training and validation cohorts. (A) Gene feature selection using the least absolute shrinkage and selection operator (LASSO) logistic regression model; (B) Scatterplot matrix of the interrelationship among selected genes after dimensionality reduction; (C) Heatmap of nine selected genes and clinical features; (D) Receiver operating characteristic curves of the gsTLS for predicting the TLSs in the training cohort, internal validation cohort, and external validation cohort; (E) the output gsTLS score of different TLSs status in the training cohort, internal validation cohort, and external validation cohort. gsTLS, gene signature of tertiary lymphoid structures; H&E, hematoxylin-and-eosin; TLSs, tertiary lymphoid structures.