. 2024 Oct 10;109:239–263. doi: 10.3897/mycokeys.109.134136

Table 2.

Details of genetic markers with PCR primers and thermal cycling program for PCR amplification.

Genetic Marker	Primers	PCR thermal cycle protocols	References
The 18S small subunit rDNA (SSU)	NS1	^aAnnealing at 55 °C for 15 s^c	White et al. (1990)
The 18S small subunit rDNA (SSU)	NS4		White et al. (1990)
The 28S large subunit rDNA (LSU)	LR0R		Rehner and Samuels (1994)
The 28S large subunit rDNA (LSU)	LR5		Vilgalys and Hester (1990)
The internal transcribed spacers (ITS)	ITS5		White et al. (1990)
The internal transcribed spacers (ITS)	ITS4		White et al. (1990)
The translation elongation factor 1-alpha (tef1-α)	EF1-983F	^aAnnealing at 55 °C for 30 s^c	Rehner and Buckley (2005)
The translation elongation factor 1-alpha (tef1-α)	EF1-2218R	^aAnnealing at 55 °C for 30 s^c	Rehner and Buckley (2005)
The partial RNA polymerase second largest subunit (rpb2)	fRPB2-5F	^bAnnealing at 57 °C for 50 s^c	Liu et al. (1999)
The partial RNA polymerase second largest subunit (rpb2)	fRPB2-7cR	^bAnnealing at 57 °C for 50 s^c	Liu et al. (1999)

Notes: ^a initial denaturation at 94 °C for 3 min, followed by 35 cycles of denaturation at 94 °C for 10 s, elongation at 72 °C for 20 s; ^b initial denaturation at 95 °C for 3 min, followed by 35 cycles at 95 °C for 45 s, elongation at 72 °C for 1.5 min; ^c final extension at 72 °C for 10 min.