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. 2024 Oct 17;7:1342. doi: 10.1038/s42003-024-07042-3

Fig. 10. PK2 KtoA mutants have elevated levels of UCP-4 protein but not mRNA.

Fig. 10

a Representative western blot reacted with anti-HA to detect UCP-4-HA. An HA tag was inserted at the C-terminus of UCP-4 by CRISPR/Cas9 in a wild-type background and crossed into the unc-89 mutants. b Quantitation of western blot results with n = 3–5. Statistical significance was assessed using an unpaired t-test with Welch’s correction. c Results of quantitative real-time PCR comparing levels of ucp-4 mRNA in wild type vs. the unc-89 mutants. Statistical significance was assessed using an unpaired t-test with Welch’s correction.