Figure 4.

Tissue- and sex-specific mRNA targets negatively corelated with miR-374 expression. In the same mouse samples used to quantify tissue-specific expression of miR-374, we found a female-specific cluster of mRNA target overexpression in the white adipose tissue of female PP mice compared with female controls and male PP mice (HIF1A, NUMB, TGFBR2), whereas male PP mice showed overexpression of the mRNA target CRK-II compared with female PP mice (A). In the spinal cord, where male PP mice show significantly elevated levels of miR-374 compared with female PP mice, male mice show marked downregulation of the predicted mRNA binding partners ATXN7 and TGFBR2 (B). Data from female mice and their derived cultures are shown in pink, whereas male mice are shown in black. Data are mean +SEM, and asterisk is P < 0.05 by Tukey's HSD compared with same sex control, while # is P < 0.05 by Tukey's HSD compared with PP group of the opposite sex. PP, primary pain; HSD, Honestly Significant Difference.