Figure 5.

miR-374 inhibits neuron activity. Using primary DRG cultures from Pirt-GCaMP3 mice, measurements of calcium signaling following a capsaicin challenge, a proxy for neuron activity, were collected. Wells were either not transfected (naive) or transfected with a nonsense sequence negative control (scramble) or an mmu-miR-374b-5p mimic (miR-374b). miR-374b treatment significantly lowered the percentage of neurons that reached an activation threshold of ≥20% compared with baseline recordings (A) and reduced average maximum fluorescent intensity (B). miR-374b treatment group has significantly lower calcium signaling compared with the other treatments (C), illustrated in a tracing plot (D). Data from female-derived cultures are shown in pink, whereas male-derived cultures are shown in black. Data are mean +SEM, and asterisk indicates a Tukey's HSD P < 0.05. DRG, dorsal root ganglia; HSD, Honestly Significant Difference.