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. 2024 Oct 17;15:8974. doi: 10.1038/s41467-024-53306-1

Fig. 3. cFLIPtDED double-layer intermediate complex utilizes different CBS to recruit Casp-8tDED and cFLIPtDED.

Fig. 3

a Crystal structure of the binary CF-C8FGLG complex. Please refer to the legend of Fig. 1c for details on the molecular color schemes used. b Different CBS in the binary CF-C8FGLG complex. The green dashed box shows the C8 tetramer portion, while the blue box shows the CF portion. Orange lines highlight the impaired CBS on the C8FGLG tetramer due to a space, indicated by a red arrow, created by the absence of FADD DED. Red lines indicate the potential CBS on the WT CF double-layer intermediate complex. The CF and C8FGLG portions are separated for clarity in visualizing the CBS. c Displays the conserved type III-II-III CSS-mediated CF and C8FGLG trimers from (b) and (d), respectively. Molecules with the same chain ID in (b) to (d) are oriented similarly. d The green dashed box highlights the CBS, represented by orange lines, on the FADD-Casp-8 intermediate complex of our previous ternary complex in Fig. 1d for comparing with the CBS in (b). For clarity, the FADD-C8FGLG and CF portions are separated. The CF portion is in the blue box. e, f Red lines and orange lines highlight the CBS for the Casp-8 molecule C8a and the cFLIP molecule CFo, respectively, on the WT CF double-layer intermediate complex in (b), viewed from different angles. g 2D representation of the binary complexes illustrates distinct CBS, extending the complex along the C8 (top) end and CF (bottom) end. A notable distinction between the CFH7G-C8FGLG and CF-C8FGLG complexes is the presence of additional cFLIPtDED molecules CFo, CFm, and CFn in the latter. Each hexagon represents a DED domain, while the dashed ones represent the adjacent tDEDs from the other layers. As in (b), a red arrow indicates a space created by the absence of FADD DED. Red lines and orange lines highlight different CBS described in (e) and (f). Pink and green angled lines are detailed in Supplementary Fig. 3. C8d1 denotes DED1 of Casp-8tDED chain d, while CFf represents cFLIPtDED chain (f).