Accuracy (N = 3) and precision results – repeatability (N = 6 samples per concentration level in one day) and intermediate precision (N = 9 samples per concentration level in 3 days).

ALP concentration (μg mL−1)	Accuracy	Repeatability		Intermediate precision
	Bias (%)	Migration time RSD (%)	IS-corrected peak area RSD (%)	Migration time RSD (%)	IS-corrected peak area RSD (%)
40	3.0	0.08	1.25	1.73	4.96
50	0.9	0.17	0.67	1.00	3.93
60	2.3	0.29	1.10	0.31	5.87