Figure 5.

NET-dependent enhancement of cell migration. Untreated or NET-treated H1975 cells were seeded in the upper compartment of transwell and allowed to migrate for 16 h. No chemoattractant, 3% FBS or 10%FBS was added to the medium in the lower compartment. On the left, representative images showing migrated cells (100X Magnification) obtained with no FBS (A), 3% FBS (B) and 10% FBS (C) in the lower compartment. On the right, the corresponding graph showing the mean number/field (± SE) of migrated cells obtained in each condition. Cell migration was 1.7 (A), 2.1 (B), and 2.7 (C) folds higher in NET-treated cells as compared to untreated cells when medium without serum, or supplemented with 3% FBS and 10% FBS, respectively, were used as chemoattractant. Statistical significance: *p<0.05.