Figure 6.

Levels of signaling mediators in EGFR-driven HCC827 and H1975 cells exposed to NETs. (A) Levels of p-ILK, ILK and CDC42 in cells exposed or not to 0.5 µg/mL NETs for 4 h, 24 h and 48 h. (B) Levels of transcription factors in adherent cells exposed or not to treatment with NETs for 4 h, 24 h and 48 h (lanes 2,4,6) and in viable cells that were detached after 48 h treatment with NETs (lane 7, +D). (C) Levels of total Notch 1 and cleaved Notch 1 in HCC827 and H1975 cells exposed or not to NETs for 4 h, 24 h and 48 h. GAPDH and vinculin were used as equal loading.