Figure 3.
Common mRNA targets of ERα and PARN suggests functional overlap in regulating nuclear deadenylation and gene expression in a cell specific manner. A-C) endogenous common mRNA transcripts of ERα and PARN analysed by qRT-PCR using RNA samples from breast cancer cells depleted of ERα (A–B, left panels) or PARN (A–C, right panels) by siRNA treatment. The levels of transcripts observed for each condition were normalized to the levels of ubiquitin C (UBC) from cells treated with control (CTRL) siRNA. Fold changes were calculated using the ΔΔCT method. Left panel, C) basal endogenous transcripts analysed by qRT-pcr using RNA samples from untreated ERα(-) breast cancer cells were normalized to UBC. Representative qRT-PCR analysis from at least three independent biological samples analysed as triplicates is shown. Experiments with two groups were analysed using two-tailed unpaired Student’s t-test. The p-values are indicated as *(<0.01), **(<0.001), ***(<0.0001) and ****(<0.00001).