FIG. 2.

Translocation of ICP0 from the nucleus to the cytoplasm in HEL fibroblasts infected with wild-type virus is blocked by PAA. Replicate slide cultures of HEL fibroblasts were infected with 20 PFU of HSV-1(F), R7914, or d120 mutant virus per cell in the presence or absence of 300 μg of PAA per ml of medium. The cells were fixed at 12 h after infection and reacted with polyclonal rabbit antibody against ICP0 and then with FITC-conjugated goat anti-rabbit IgG. The tabulation of cells exhibiting ICP0 in the nucleus, the cytoplasm, or both was done as described in the legend to Fig. 1.