FIG. 5.

Effect of MG132 proteasomal inhibitor on the translocation of ICP0 from the nucleus to the cytoplasm. Replicate slide cultures of HEL fibroblasts were exposed to 20 PFU of HSV-1(F) or d120 mutant virus per cell. At 2 h after infection (middle bars) or 9 h after infection (right bars), the cells were replenished with medium containing MG132 (5 μM). At 12 h after infection, the cells were fixed and reacted with polyclonal rabbit antibody against ICP0 and then with goat anti-rabbit IgG conjugated to FITC. The cells were examined and quantified as described in the legend to Fig. 1.