FIG. 6.

Colocalization of proteasome and ICP0 in cells infected with the d120 mutant and exposed to MG132. The experimental design of this series of experiments was identical to that described in the legend to Fig. 3, except that the cells were reacted with mouse monoclonal antibody against ICP0 and rabbit polyclonal antibody to the whole proteasome designated as core antibody (Affiniti Research Products Ltd.). The secondary antibodies were goat anti-mouse IgG conjugated to FITC and goat anti-rabbit IgG conjugated to Texas Red. Shown are images of cells infected with d120 mutant virus and either left untreated (upper panels) or exposed at 9 h after infection to 5 μM MG132 (lower panels). The images were captured with a Zeiss confocal microscope with the aid of software provided by the manufacturer. The arrows point to proteasomal core proteins aggregated in the form of a shell surrounding ICP0 in the cytoplasm of MG132-treated cells. These structures were seen only in d120 mutant-infected cells treated with MG132.