Figure 4.

Hx alleviates perivascular edema in ischemic lungs of the unilateral pulmonary IRI mouse model. (A) Levels of perivascular edema were evaluated histologically by assessing five vessels of right and left lungs, respectively. Distances from tunica intima to tunica intima (Dv) were compared with the shortest diameter from tunica adventia to tunica adventia (Dc). Shortest distances were utilized as measured values to compensate for distortion of the results caused by different cutting planes. To compare different vessel sizes, the results were combined into one parameter with the formula cuff rea/vessel area = D_c ²-D_V ²/D_V ², as indicated. (B) The extent of perivascular edema, as determined by the cuff area-to-vessel area ratio, was markedly higher in the left ischemic compared to the right control lung (10.6 (5.7) vs. 6.5 3.4), respectively: p = 0.031). Increased levels of cuff area-to-vessel area ratio in ischemic lungs were reduced by treatment with Hx (IRI group vs. Hx treated group: 10.6 (5.7) vs. 5.9 (2.7), respectively: p = 0.032). n=5 mice for the vehicle group (IRI and Con), and, n=5 mice for the Hx-treated group (IRI+Hx and Con+Hx). (Outlier [35.4] in the IRI with vehicle group was excluded). N.S., not significant.