Figure 7.

Hx affects levels of immune cells in peripheral blood from mice with unilateral lung IRI. The gating strategy for flow cytometry analysis of various immune cells is described in Materials and Methods. (A, B) Significant lower levels of B- (CD3⁺CD19⁺) and CD8+ T (CD3⁺CD8⁺) cells were detected in IRI mice with Hx compared to mice without Hx treatment (n = 5 mice in each group: p=0.037 and 0.008, respectively). (C) Significantly higher levels of dendritic cells were found in IRI mice with Hx treatment than mice without Hx treatment (n = 5, p=0.04).