Figure 7.

5c(ii) promotes neuroprotection. Primary adult cortical neurons were extracted from 6-week-old male mice and plated on 384-well PDL-coated plastic bottom plates at 10,000 cells/well. 5c(ii) at 5 μM concentration was added at the time of plating. 48 h after plating, the cells were fixed to conduct immunocytochemistry. Graph represents the mean ± SEM of (a) total neurite outgrowth per cell and (b) number of valid neurons per well. Representative images of cortical neurons treated with (c) vehicle and (d) 5 μM 5c(ii). Student’s t test. N = 3–5. *p < 0.05.