Figure 2.

Unveiling the melodious interplay of Linc00513, miR-7, and TGFBR1 in the silencing symphony of spermatogenesis. A) qRT-PCR analysis of Linc00513 expression in TCAM-2(Azoospermia cell line) and HS1 (Normal cell line) cell lines. The expression of Linc00513 was significantly lower in TCAM-2 cells compared to HS1 cells, suggesting a potential role for Linc00513 in the regulation of spermatogenesis; B) qRT-PCR analysis of miR-7 expression in TCAM-2 and HS1 testes. The expression of miR-7 was significantly higher in TCAM-2 testes compared to HS1 testes, suggesting an inverse relationship between Linc00513 and miR-7 expression in spermatogenesis; C) Computational analysis of miR-7 binding sites in the TGFBR1 gene. A predicted binding site for miR-7 was identified within the 3’ UTR of the TGFBR1 gene, suggesting that miR-7 could potentially regulate TGFBR1 expression and activity.