FIG. 6.

Intracellular localization patterns of mutant IE2 proteins unable to be modified by SUMO-1. Vero cells were transfected with pJHA124 encoding wild-type IE2 (a and b), pYX105 encoding IE2(K175R) (c and d), pYX106 encoding IE2(K180R) (e and f), or pYX104 encoding IE2(K175/180R) (g and h). At 48 h after transfection, the cells were fixed by the paraformaldehyde procedure and double-label IFA was carried out with MAb 12E2 for IE2 (a, c, e, and g) or PAb PML(C) for endogenous PML (b, d, f, and h). FITC-labeled anti-mouse IgG and rhodamine-coupled anti-rabbit IgG were used for visualization.