Figure 5.

Peptide degradation data in freeze–thaw lysates. (a) Representative electropherograms of peptides incubated in vegetative cell lysates from T. thermophila B2086.2 (lighter) and D. discoideum (darker). Time points were selected to represent roughly one-half-life for each peptide (i.e., when the full-length reporter was approximately 50% degraded). Numbered peaks indicate peptide fragments identified by spiking with known standards and represented by the number of N-terminal amino acids remaining from the original unmodified reporter. (b-d) Degradation of the reporters over time in lysates from (b) D. discoideum, (c) T. thermophila B2086.2, and (d) T. thermophila CU428.2. Error bars represent the standard deviation for n = 3 biological replicates.