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. 2024 Oct 18;22:634. doi: 10.1186/s12951-024-02890-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 10 — Immunohistochemical (IHC) stainings of nucleus pulposus tissues in rats at 4 and 8 weeks after treatment. A Immunohistochemical staining of protein ACAN, COLIIA1, GPX1 and IL-1β of rats’ IVDs at 8 weeks after treatment. B Quantification of the percentages of ACAN-positive and COL2A1-positive area in nucleus pulposus tissue. C Quantification of the percentages of GPX1-positive and IL-1β-positive cells in nucleus pulposus tissue. Statistically significant differences are indicated by # where P < 0.05 or ## where P < 0.01 compared with the control group; * where P < 0.05 or ** where P < 0.01 between the indicated group