Fig. 3.

Effect of SeNPs on mitochondrial function of normal NPCs. NPCs were treated with SeNPs concentrations of 25,50 and 100 ng/mL. A Transmission electron microscopy images of intracellular mitochondria of NPCs. B Effect of SeNPs on ATP content in NPCs. C JC-1 fluorescence staining results of NPCs under different concentrations of intervention of SeNPs. D Quantitative RT-PCR was used to determine the mRNA levels of genes Sdha, Atp5, Nd4 and Cox4. (n = 4) E Western blotting was used to determine the effects of the SeNPs on the levels of protein SDHA, ATP5, ND4 and COX4 in NPCs. Statistically significant differences are indicated by # where P < 0.05 or ## where P < 0.01 compared with the control group; * where P < 0.05 or ** where P < 0.01 between the indicated group