TABLE 1.
Reporter cassette | No. of neurons countedc (% β-gal-expressing neurons)d on day p.i.
|
|||
---|---|---|---|---|
1 | 3 | 4 | 6 | |
CMV > lacZb | 127 (58) | 375 (46) | ND (ND)f | 246 (10) |
IE110 > lacZe | 341 (54) | 281 (67) | 285 (19) | 287 (1) |
Neuronal cultures infected with 106 PFU of either CS5 or gH−TK−IE110LacZ per well.
CMV IE1 reporter promoter driving lacZ expression in CS5-infected wells.
One well per data point was fixed and dually immunostained for β-gal and β-tub, and the number of neurons (β-tub+) was counted.
Percentage of neurons showing β-gal expression.
IE110 reporter promoter driving lacZ expression in gH−TK−IE110LacZ-infected wells.
ND, not determined.