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. 2001 Apr;75(8):3885–3895. doi: 10.1128/JVI.75.8.3885-3895.2001

TABLE 1.

Activation of CMV and IE110 promoters on initial infection of neuronal culturesa

Reporter cassette No. of neurons countedc (% β-gal-expressing neurons)d on day p.i.
1 3 4 6
CMV > lacZb 127 (58) 375 (46) ND (ND)f 246 (10)
IE110 > lacZe 341 (54) 281 (67) 285 (19) 287 (1)
a

Neuronal cultures infected with 106 PFU of either CS5 or gHTKIE110LacZ per well. 

b

CMV IE1 reporter promoter driving lacZ expression in CS5-infected wells. 

c

One well per data point was fixed and dually immunostained for β-gal and β-tub, and the number of neurons (β-tub+) was counted. 

d

Percentage of neurons showing β-gal expression. 

e

IE110 reporter promoter driving lacZ expression in gHTKIE110LacZ-infected wells. 

f

ND, not determined.