TABLE 3.

VC1-infected neuronal cultures^a

Reporter cassette	Days p.i.b	Total no. of neurons/wellc	No. of positive neurons/welld	% Positive neuronse
CMV>GFPf	3	897	440	49
		574	367	64
CMV>GFP	14	789	13	2
LAP>β-galg	14	693	238	34
		506	231	47
		795	280	35

^aNeuronal cultures infected with 10⁶ PFU of VC1 per well. 

^bFor enumeration, wells were fixed at the stated times p.i. and were immunostained for β-tub alone or β-tub and β-gal. 

^cTotal number of neurons (β-tub⁺ cells) in the wells examined. 

^dTotal number of neurons per well showing staining for reporter gene expression. 

^ePercentage of neurons in the well showing reporter gene expression. 

^fCMV IE1 promoter-driven expression of GFP. 

^gLAP-driven expression of β-gal.