TABLE 4.

VC1-infected cultures on different days p.i.

Day p.i.	Stimulus	Total no. of neurons/well	GFP+/well prereactivationa	GFP+/well after reactivationb	% Positive neurons
15	Normal mediumc	845	18	19	2
		758	55	42	6
	NGF−d	389	38	264	68
		327	18	103	31
	TSA+e	941	50	213	26
28	Normal medium	655	NAf	9	1
		657	NA	69	10
	NGF−	334	NA	106	31
		262	NA	101	39
	TSA+	653	NA	213	33
		1,012	NA	418	39

^aNumbers of GPP⁺ neuron-like cells (identified by morphology) in living cultures prior to replacement of culture medium on day 14 p.i. 

^bValues in top half of table reflect number of GFP⁺ neurons per well 24 h after replacement of culture medium (i.e., day 15), which was enumerated from fixed cultures immunostained for β-tub. Values in lower half reflect number of GFP⁺ neurons per well on day 28 p.i. 24 h after replacement of culture medium. 

^cCulture medium was replaced with fresh normal growth medium. 

^dCulture medium was replaced with fresh growth medium lacking NGF and containing 0.1 pl of rabbit anti-NGF antiserum/ml. 

^eCulture medium was replaced with fresh growth medium supplemented with 660 nM TSA. 

^fNA, not applicable.