TABLE 5.

Activation of CMV and IE110 promoters in latently infected cultures^a

Reporter cassette	Stimulusef	No. of neurons countedb (% β-gal+)c
CMV>lacZd	Normal feed	187 (2)
	TSA+	163 (53)
IE110>lacZg	Normal feed	189 (2)
	TSA+	397 (18)

^aNeuronal cultures 22 days after infection with 10⁶ PFU of either CS5 or gH⁻TK⁻IE110LacZ per well. They were fixed and dually immunostained for β-gal and β-tub. 

^bTotal neurons (β-tub⁺) examined (two wells per data point). 

^cPercentage of neurons showing β-gal expression. 

^dCMV IE1 promoter-driven lacZ expression in CS5-infected wells. 

^eCulture medium for normal feed was replaced with fresh growth medium on day 21 p.i. 

^fCulture medium for TSA⁺ was replaced with fresh growth medium supplemented with 660 nM TSA on day 21 p.i. 

^gIE110 promoter-driven lacZ expression in gH⁻TK⁻IE110LacZ-infected wells.