Fig. 1.

Effect of TLR ligands in diaphragmatic hernia induced by nitrofen. A Pregnant Wistar rat females were administered nitrofen (n = 6), or olive oil as placebo (control) (n = 2), at E9.5 and 3 days later injected intraperitoneally with 100 μg/Kg of the indicated immunomodulators or only saline (None or Untreated). Results are represented as percentages of all fetuses pregnant rats with hernia (CDH +) at E21. B and C Pregnant Wistar rat females were administered nitrofen at E9.5 and 5 h, 1 day or 3 days later injected intraperitoneally with 100 μg/Kg of CS1 or saline. B Diaphragmatic hernia percentage. Results are represented as percentages of all fetuses with hernia (CDH +) at E21. C The weight of the fetuses recorded at E21. **p > 0.001, *p > 0.01 respect to untreated nitrofen group (control). In this case, all fetuses from 3 rats from each group, independent or not of the presence of hernia, were analyzed