Fig. 5.

Immune infiltration in lung and diaphragm induced by nitrofen and CS1 treatments. Pregnant Wistar rat females (3 per group) were treated with nitrofen or olive oil (control) at E9.5 and 3 days later injected intraperitoneally or with 100 µg/Kg CS1 or saline. A–D Indicated cell infiltration in the diaphragm, left and right lung was determined by immunohistochemistry at E21. In the CS1 treated groups, both CDH-negative and CDH-positive fetuses were analysed separately. E CD206 expression was determined by immunofluorescence at E18. **p > 0.001 and *p > 0.01, respect to control normal group; ☨☨p > 0.001 and ☨p > 0.01 respect to untreated CDH + nitrofen group