Fig. 2.

U0126 inhibits liraglutide-induced neurite outgrowth of cortical neurons. a Presentative appearance of cortical neuron with different treatment. a Control: neurons cultured with 0.1 % DMSO. b Neurons cultured with 10-μM U0126. c Neurons cultured with 100-nM liraglutide d Neurons cultured with 100-nM liraglutide and 10-μM U0126. Bar 20 μm. b Statistic results in different groups were shown as above chart. Values represent the mean ± SD (n = 60 neurons). Asterisk represents statistic difference. P < 0.05 using one-way ANOVA