Figure 1.

Determination of the Minimum Effective Dose. Representative images of ICG fluorescence in vivo (A) and ex vivo in the tumor, lung, and liver (B), obtained with the IVIS Spectrum Imaging System and the SPY Elite intraoperative imaging device. The tumor margins in the in vivo pictures are highlighted by the green dotted line. In the color scale, blue represents the minimum fluorescence signal, whereas red represents the maximum. Additional ex vivo images from other replicates are presented in Supplementary Figure 3. Mice bearing tumors were administered three dosages of nanoparticles - 3.3E09 EVs/Kg, 3.3E08 EVs/Kg, or 3.3E07 EVs/Kg - of the ONCOGREEN formulation. C) Quantification of the fluorescent signals in the tumors 24 hours after injection is presented in the graph; bars in the graph represent the average +/- S.E.M values of eight animals, *** p < 0.001, ** p < 0.01 calculated by one-way ANOVA followed by Bonferroni's test.