Table 1.

Nucleotide sequences of the oligonucleotide primers and optimized conditions for the amplification of P. falciparum K13 gene

Reaction	Primer name	Primer sequence 5'– > 3'	PCR product length	Cycling conditions
First round PCR (outer)	PfK13.F3	AGTGGAAGACATCATGTAACCAG	1143 bp	Denaturation 94˚C for 5 min, then 35 cycles of 94 ˚C for 30 s, 55 ˚C for 30 s, 68 ˚C for 1 min 30 s, and a final elongation of 68 ˚C for 5 min
	PfK13.R3	TGTGCATGAAAATAAATATTAAAGAAG
Second round PCR (inner semi-nested)	PfK13.F3	AGTGGAAGACATCATGTAACCAG	1021 bp
	PfK13.R2	CGGAGTGACCAAATCTGGG
Sequencing primers	PfK13.F1 PfK13.R1	GCCTTGTTGAAAGAAGCAGAA GCCAAGCTGCCATTCATTTG