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. 2024 Oct 18;22:640. doi: 10.1186/s12951-024-02898-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Increase of activation markers and specific killing after interaction of unactivated AbC T cells and target cells. Expression of CD69 (A), CD25 (B), forward scatter profile (C), perforin (D) and granzyme (E) on IgG control AbC T cells or 14F7hT AbC T cells was measured by flow cytometry after co-culturing with antigen-expressing L1210 cells at 4:1 E/T ratio. F Control and AbC T cells were evaluated via co-immunoprecipitation for 14F7hT linkage to CD3. The input and eluted materials were evaluated by Western blot. G T or AbC T cells were cultured with antigen-expressing L1210 cells for 16 h at different E/T ratios. Cells were stained with 7-AAD and analyzed by flow cytometry with target cells analyzed for their death rates. Flow cytometry data is representative of 3 independent experiments with T cells sourced from 3 different donors. Data represents the mean ± SD (n = 3). (Student’s t-test, **p < 0.01, ***p < 0.001 and ****p < 0.0001, ns not significant)