FIG. 4.
Identification of an OBPH7-binding site in the HHV-7 oriLyt. (A) Schematic diagram of the genomic location of the 600-bp HHV-7 minimal oriLyt region (27). The triangles indicate the putative HHV-7 OBP-2 and OBP-1 sites. (B) The 18 double-stranded 55-bp oligonucleotides (1 to 18) shown in panel A were used for EMSA. 32P-labeled oligonucleotides were reacted with IVTT lysate programmed with truncated U73 in the reverse orientation (negative control, left lane for each oligonucleotide) or with truncated U73 in the correct forward orientation (right lane for each oligonucleotide). Asterisks indicate oligonucleotides with the putative OBP-2 and OBP-1 sites. Oligonucleotide 13 labeled inefficiently to lower specific activity; no specific binding was detected upon long exposure or by PhosphorImager analysis (data not shown). The arrowhead indicates the complex generated in the presence of oligonucleotide 4 and OBPH7-containing IVTT.