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. 1988 Feb 15;250(1):179–188. doi: 10.1042/bj2500179

The effects of surgical stress and short-term fasting on protein synthesis in vivo in diverse tissues of the mature rat.

V R Preedy 1, L Paska 1, P H Sugden 1, P S Schofield 1, M C Sugden 1
PMCID: PMC1148830  PMID: 2451506

Abstract

1. We measured fractional rates of protein synthesis, capacities for protein synthesis (i.e. RNA/protein ratio) and efficiencies of protein synthesis (i.e. protein-synthesis rate relative to RNA content) in fasted (24 or 48 h) or fasted/surgically stressed female adult rats. 2. Of the 15 tissues studied, fasting caused decreases in protein content in the liver, gastrointestinal tract, heart, spleen and tibia. There was no detectable decrease in the protein content of the skeletal muscles studied. 3. Fractional rates of synthesis were not uniformly decreased by fasting. Rates in striated muscles, uterus, liver, spleen and tibia were consistently decreased, but decreases in other tissues (lung, gastrointestinal tract, kidney or brain) were inconsistent or not detectable, suggesting that, in many tissues in the mature rat, protein synthesis was not especially sensitive to fasting. 4. In fasting, the decreases in fractional synthesis rate resulted from changes in efficiency (liver and tibia) or from changes in efficiency and capacity (heart, diaphragm, plantaris and gastrocnemius). In the soleus, the main change was a decrease in capacity. 5. Surgical stress increased fractional rates of protein synthesis in diaphragm (where there were increases in both efficiency and capacity) by about 50%, in liver by about 20%, in spleen by about 40%, and possibly also in the heart. In liver and spleen, capacities were increased. In other tissues (including the skeletal muscles), the fractional rates of protein synthesis were unaffected by surgical stress.

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Selected References

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  1. Augustine S. L., Swick R. W. Turnover of total proteins and ornithine aminotransferase during liver regeneration in rats. Am J Physiol. 1980 Jan;238(1):E46–E52. doi: 10.1152/ajpendo.1980.238.1.E46. [DOI] [PubMed] [Google Scholar]
  2. Brown J. G., van Bueren J., Millward D. J. The effect of tri-iodothyronine administration on protein synthesis in the diabetic rat. Biochem J. 1983 Aug 15;214(2):637–640. doi: 10.1042/bj2140637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clague M. B., Keir M. J., Wright P. D., Johnston I. D. The effects of nutrition and trauma on whole-body protein metabolism in man. Clin Sci (Lond) 1983 Aug;65(2):165–175. doi: 10.1042/cs0650165. [DOI] [PubMed] [Google Scholar]
  4. Cuthbertson D. P. The disturbance of metabolism produced by bony and non-bony injury, with notes on certain abnormal conditions of bone. Biochem J. 1930;24(4):1244–1263. doi: 10.1042/bj0241244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Frayn K. N. Hormonal control of metabolism in trauma and sepsis. Clin Endocrinol (Oxf) 1986 May;24(5):577–599. doi: 10.1111/j.1365-2265.1986.tb03288.x. [DOI] [PubMed] [Google Scholar]
  6. Garlick P. J., Fern M., Preedy V. R. The effect of insulin infusion and food intake on muscle protein synthesis in postabsorptive rats. Biochem J. 1983 Mar 15;210(3):669–676. doi: 10.1042/bj2100669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garlick P. J., McNurlan M. A., Preedy V. R. A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine. Biochem J. 1980 Nov 15;192(2):719–723. doi: 10.1042/bj1920719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garlick P. J., Millward D. J., James W. P., Waterlow J. C. The effect of protein deprivation and starvation on the rate of protein synthesis in tissues of the rat. Biochim Biophys Acta. 1975 Nov 18;414(1):71–84. doi: 10.1016/0005-2787(75)90126-4. [DOI] [PubMed] [Google Scholar]
  9. Goldspink D. F., Kelly F. J. Protein turnover and growth in the whole body, liver and kidney of the rat from the foetus to senility. Biochem J. 1984 Jan 15;217(2):507–516. doi: 10.1042/bj2170507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goldspink D. F., Lewis S. E., Kelly F. J. Protein synthesis during the developmental growth of the small and large intestine of the rat. Biochem J. 1984 Jan 15;217(2):527–534. doi: 10.1042/bj2170527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goodman M. N., Ruderman N. B. Starvation in the rat. I. Effect of age and obesity on organ weights, RNA, DNA, and protein. Am J Physiol. 1980 Oct;239(4):E269–E276. doi: 10.1152/ajpendo.1980.239.4.E269. [DOI] [PubMed] [Google Scholar]
  12. Lewis S. E., Kelly F. J., Goldspink D. F. Pre- and post-natal growth and protein turnover in smooth muscle, heart and slow- and fast-twitch skeletal muscles of the rat. Biochem J. 1984 Jan 15;217(2):517–526. doi: 10.1042/bj2170517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McNurlan M. A., Garlick P. J. Contribution of rat liver and gastrointestinal tract to whole-body protein synthesis in the rat. Biochem J. 1980 Jan 15;186(1):381–383. doi: 10.1042/bj1860381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McNurlan M. A., Garlick P. J. Protein synthesis in liver and small intestine in protein deprivation and diabetes. Am J Physiol. 1981 Sep;241(3):E238–E245. doi: 10.1152/ajpendo.1981.241.3.E238. [DOI] [PubMed] [Google Scholar]
  15. Millward D. J., Garlick P. J., James W. P., Nnanyelugo D. O., Ryatt J. S. Relationship between protein synthesis and RNA content in skeletal muscle. Nature. 1973 Jan 19;241(5386):204–205. doi: 10.1038/241204a0. [DOI] [PubMed] [Google Scholar]
  16. Millward D. J., Nnanyelugo D. O., James W. P., Garlick P. J. Protein metabolism in skeletal muscle: the effect of feeding and fasting on muscle RNA, free amino acids and plasma insulin concentrations. Br J Nutr. 1974 Jul;32(1):127–142. doi: 10.1079/bjn19740063. [DOI] [PubMed] [Google Scholar]
  17. Moldawer L. L., O'Keefe S. J., Bothe A., Jr, Bistrian B. R., Blackburn G. L. In vivo demonstration of nitrogen-sparing mechanisms for glucose and amino acids in the injured rat. Metabolism. 1980 Feb;29(2):173–180. doi: 10.1016/0026-0495(80)90143-2. [DOI] [PubMed] [Google Scholar]
  18. Müller M. J., Seitz H. J. Pleiotypic action of thyroid hormones at the target cell level. Biochem Pharmacol. 1984 May 15;33(10):1579–1584. doi: 10.1016/0006-2952(84)90277-6. [DOI] [PubMed] [Google Scholar]
  19. Odedra B. R., Bates P. C., Millward D. J. Time course of the effect of catabolic doses of corticosterone on protein turnover in rat skeletal muscle and liver. Biochem J. 1983 Aug 15;214(2):617–627. doi: 10.1042/bj2140617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pomposelli J. J., Palombo J. D., Hamawy K. J., Bistrian B. R., Blackburn G. L., Moldawer L. L. Comparison of different techniques for estimating rates of protein synthesis in vivo in healthy and bacteraemic rats. Biochem J. 1985 Feb 15;226(1):37–42. doi: 10.1042/bj2260037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Powanda M. C., Wannemacher R. W., Jr, Cockerell G. L. Nitrogen metabolism and protein synthesis during pneumococcal sepsis in rats. Infect Immun. 1972 Sep;6(3):266–271. doi: 10.1128/iai.6.3.266-271.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Preedy V. R., Garlick P. J. The effect of glucagon administration on protein synthesis in skeletal muscles, heart and liver in vivo. Biochem J. 1985 Jun 15;228(3):575–581. doi: 10.1042/bj2280575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Preedy V. R., McNurlan M. A., Garlick P. J. Protein synthesis in skin and bone of the young rat. Br J Nutr. 1983 May;49(3):517–523. doi: 10.1079/bjn19830060. [DOI] [PubMed] [Google Scholar]
  24. Preedy V. R., Smith D. M., Kearney N. F., Sugden P. H. Rates of protein turnover in vivo and in vitro in ventricular muscle of hearts from fed and starved rats. Biochem J. 1984 Sep 1;222(2):395–400. doi: 10.1042/bj2220395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Preedy V. R., Smith D. M., Sugden P. H. The effects of 6 hours of hypoxia on protein synthesis in rat tissues in vivo and in vitro. Biochem J. 1985 May 15;228(1):179–185. doi: 10.1042/bj2280179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rennie M. J. Muscle protein turnover and the wasting due to injury and disease. Br Med Bull. 1985 Jul;41(3):257–264. doi: 10.1093/oxfordjournals.bmb.a072060. [DOI] [PubMed] [Google Scholar]
  27. Sakamoto A., Moldawer L. L., Palombo J. D., Desai S. P., Bistrian B. R., Blackburn G. L. Alterations in tyrosine and protein kinetics produced by injury and branched chain amino acid administration in rats. Clin Sci (Lond) 1983 Mar;64(3):321–331. doi: 10.1042/cs0640321. [DOI] [PubMed] [Google Scholar]
  28. Schofield P. S., French T. J., Sugden M. C. Ketone-body metabolism after surgical stress or partial hepatectomy. Evidence for decreased ketogenesis and a site of control distal to carnitine palmitoyltransferase I. Biochem J. 1987 Jan 15;241(2):475–481. doi: 10.1042/bj2410475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Stein T. P., Leskiw M. J., Wallace H. W., Oram-Smith J. C. Changes in protein synthesis after trauma: importance of nutrition. Am J Physiol. 1977 Oct;233(4):E348–E355. doi: 10.1152/ajpendo.1977.233.4.E348. [DOI] [PubMed] [Google Scholar]
  30. Young V. R., Huang P. C. In vivo uptake of [14C]leucine by skeletal muscle ribosomes after injury in rats fed two levels of protein. Br J Nutr. 1969 Jun;23(2):271–280. doi: 10.1079/bjn19690034. [DOI] [PubMed] [Google Scholar]

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