FIGURE 4.

We performed a mitochondrial targeted quantitative proteomics in rat gastroc and soleus skeletal muscles. The size of the dot indicates the relative abundance of the protein in the (a) gastroc and (b) solues. Visualization of the synthesis rates (K[1/day]) of the mitochondrial electron transport system complexes (CI, CII, CIII, CIV, CV) in the (c) gastroc and (d) soleus. Blue indicates a faster synthesis rate, whereas red indicates a slower synthesis rate. Gray proteins indicate that the protein was not detected in our analyses. The complexes were generated using complex IDs (CI: 7AK5, CII: 1ZOY, CIII: 7TZ6, CIV: 7COH, and CV: 8H9V) from the RCSB Protein Data Bank and rendered using ChimeraX. Data were analyzed by two‐way ANOVA (group × treatment) and are represented as mean from 5 to 7 rats per group. Within each strain $ indicates a significant difference from control group (CON), † indicates a significant difference from metformin‐treated (MET) group, and ‡ indicates significant differences from CON and MET.