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. 2001 Apr;75(8):3937–3947. doi: 10.1128/JVI.75.8.3937-3947.2001

FIG. 2.

FIG. 2

Expression of wt and mutated VP7 proteins. Sf9 cells were infected with Ac10BTV.7, expressing the wt (lane 1) or mutated (VP7-AGD [lane 2], VP7-AGQ [lane 3], and VP7-AGL [lane 4]) VP7 proteins. (A) The infected-cell lysates were analyzed by SDS-PAGE (10% polyacrylamide) and stained with Coomassie brilliant blue. (B) The proteins were electroblotted onto a membrane and reacted with BTV-10 polyclonal antisera. The arrow indicates the size of wt and mutated VP7 proteins. The protein mass markers are indicated on the left.