Fig. 1. (a) Double sandwich homologous ELISA of Ab117 reactivity (the complex is not pre-made before application to the ELISA plate) with SAT2 topotypes. SAT2/ZIM/7/83 VLPs with S2093Y and SAT2/EGY/4/2012 inactivated virus. (b) Antigen titration ELISA (starting from 3.43 µg ml−1 as 1/3 dilution) utilizing M377, M311 and Ab117 as both capture and detection antibody for the SAT2/EGY/4/2012 inactivated virus without and with heat treatment (56 °C for 15 min) to disrupt the capsids.
