Table 1. Single-particle analysis. Data collection, structure determination and refinement statistics.
| Data collection | Model refinement | ||
| Accelerating voltage (kV) | 300 | Map resolution (GSFSC=0.314; Å) | 3.2 |
| C2 aperture (µm) | 70 | D FSC model (0.5) | 3.3 |
| Energy filter width (eV) | 20 | CC1/2 (Mask) | 0.82 |
| Calibrated pixel size (Å/px) | 1.054 | Sharpening B-factor (Å2) | 51 |
| Nominal magnification (kX) | 130 | No. atoms | 21 815 |
| Dose rate (e-/Å2/s) | 9.1 | B-factors (Å2) min/max/mean | 14/185/81 |
| EER fractions | 40 | Root mean squared deviations | - |
| Total dose (e-/Å2) | 40.0 | Bond lengths (Å) | 0.003 |
| Exposure (s) | 4.4 | Bond angles (°) | 0.4 |
| Defocus range (µm) | −2.8 : −1.4 | Clash score | 5.4 |
| Movies | 13 327 | Ramachandran | - |
| Particles (final) | 110 210 | Favoured/allowed/outliers (%) | 95.3/4.7/0.0 |
| Symmetry imposed | C5 | Rotamer outlier (%) | 0.68 |