Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus, both sexes) | 129/Sj | Internal | Ref PMID:33462143 | |
| Strain, strain background (M. musculus, both sexes) | C57BL/6 J | Jackson Labs | IMSR_JAX: 000664 |
|
| Strain, strain background (M. musculus, both sexes) | Prox1-GFP BAC transgenic (Tg(Prox1-EGFP)KY221Gsat/Mmcd) | MMRRC | 031006-UCD | |
| Antibody | α-SMA (Rabbit polyclonal) | Abcam | Cat# ab5694; RRID:AB_2223021 | WM (1:50) Sections (1:200) |
| Antibody | CCL21A (Goat polyclonal) | R&D Systems | Cat# AF457-SP; RRID:AB_2072083 | WM (1:25) Sections (1:200) |
| Antibody | DARC/ACKR1 (Sheep polyclonal) | Invitrogen (Thermo Fisher Scientific) | Cat# PA5-47861; RRID:AB_2576815 | WM (1:50) Sections (1:200) |
| Antibody | Desmin (Rabbit monoclonal) | Invitrogen (Thermo Fisher Scientific) | Cat# MA532068; RRID:AB_2809362 | WM (1:50) Sections (1:200) |
| Antibody | Endomucin (Rat monoclonal) | EBioscience/Invitrogen (Thermo Fisher Scientific) | Cat# 14-5851-82; RRID:AB_891527 | WM (1:25) Sections (1:200) |
| Antibody | FLT1 (Rabbit monoclonal) | Abcam | Cat# ab32152; RRID:AB_778798 | WM (1:50) Sections (1:100) |
| Antibody | FLT4 (Goat polyclonal) | R&D Systems | Cat# AF743; RRID:AB_355563 | WM (1:50) Sections (1:200) |
| Antibody | LYVE1 (Rat monoclonal) | EBioscience/Invitrogen (Thermo Fisher Scientific) | Cat# 14-0443-80; RRID:AB_1633416 | WM (1:50) Sections (1:200) |
| Antibody | NPNT (Goat polyclonal) | R&D Systems | Cat# AF4298; RRID:AB_10645643 | WM (1:50) Sections (1:200) |
| Antibody | PECAM-1 (CD31) (Rat monoclonal) | BD Pharmingen | Cat# 550274; RRID:AB_393571 | WM (1:50) Sections (1:200) |
| Antibody | SELP (Goat polyclonal) | R&D Systems | Cat# AF737-SP, RRID: AB_2285644 |
WM (1:50) Sections (1:100) |
| Antibody | VECAD (Goat polyclonal) |
R&D Systems | Cat# AF1002; RRID:AB_2285644 | WM (1:50) Sections (1:200) |
| Commercial assay or kit | Papain Dissociation System | Worthington Biochemical | Cat# LK003153 | |
| Chemical compound, drug | 4% PFA | Thermo Fisher Scientific | Cat# 50-980-495 | |
| Chemical compound, drug | Propidium iodide | Thermo Fisher Scientific | Cat# P1304MP | |
| Chemical compound, drug | SYTOX green Nucleic Acid Stain | Thermo Fisher Scientific | Cat# S7020 | |
| Chemical compound, drug | Collagenase Type 4 | Worthington Biochemical | Cat# LS004188 | 1 mg/ml |
| Other | DAPI | Thermo Fisher Scientific | Cat# 62248 | (1:5000) Section ‘Immunofluorescence’ |
| Other | 40 and 100 μm Falcon cell strainers | Thermo Fisher Scientific | 08-771−1/08-771-19 | Section ‘Processing of tissue for single nucleus RNA sequencing’ |