Extended Data Fig. 1. Interaction test between U1 snRNP components and CBP20 and RBP47B.
a: U1-A or U1-C translationally fused to GFP was co-expressed with MYC-tagged CBP20 in Nicotiana benthamiana plants for transient protein expression. GFP alone served as a negative control. Proteins were isolated and immunoprecipitated using a GFP-affinity matrix. Input and immunoprecipitated fractions (IP) were subjected to protein blot analysis using GFP- or –MYC specific antibodies. Each experiment was repeated three times independently with similar results. b: U1-A translationally fused to RFP was co-expressed with YFP-tagged RBP47 in Nicotiana benthamiana plants for transient protein expression. RFP alone served as a negative control. Proteins were isolated and immunoprecipitated using an RFP-affinity matrix. Input and immunoprecipitated fractions (IP) were subjected to protein blot analysis using RFP- or -YFP specific antibodies. Each experiment was repeated two times independently with similar results.