Fig. 3.

ACTL8 is highly expressed in GC cell lines, and the efficiency of ACTL8 silencing and overexpression was verified in GC cells. A Histogram of ACTL8 mRNA expression measured by RT-qPCR analysis in GC tumor cells and matched nontumor cells. B Representative images of ACTL8 expression in GC cells and normal cells measured by WB. C The interference efficiency of siRNA in NCI-N87 and AGS cell lines measured by RT-qPCR. D The protein levels of ACTL8 in NCI-N87 and AGS measured by WB. E The overexpression efficiency of ACTL8 in MKN45 cell lines measured by RT-qPCR. F WB verified the efficiency of ACTL8 overexpression in MKN45 cells. GAPDH was used as the internal parameter. *p < 0.05; **p < 0.01; ***p < 0.001. GC, gastric cancer; RT-qPCR, real-time quantitative polymerase chain reaction; WB, western blotting; GAPDH, glyceraldehyde 3-phosphate dehydrogenase