Fig. 4.

ACTL8 promotes the proliferation of GC cell lines. A The proliferation of negative control (NC) cells and ACTL8 knockdown cells in NCI-N87 and AGS cell lines was determined by CCK8 assay. B Colony formation assay was executed to detect the colony numbers of the transfected NCI-N87 and AGS cells (Crystal violet staining; magnification, × 100). C CCK8 assays showed that ACTL8 overexpression promoted the growth of MKN45. D Colony formation assay was executed to detect the colony numbers of the transfected MKN45 cells (Crystal violet staining; magnification, × 100). *p < 0.05; **p < 0.01; ***p < 0.001. GC, gastric cancer; CCK8, Cell Counting Kit-8