Fig. 7.

The A393T variant does not increase susceptibility to DSS- or TNBS-mediated chemical induction of colitis. DSS was administered via the drinking water to 2–3-month-old mice, and body weight loss assessed as % of baseline was tracked for 10 days (A). Formalin-fixed, paraffin-embedded colon tissue sections were stained with hematoxylin and eosin and scored for inflammation. Representative colon images by genotype are shown in B, and the distribution of histology scores by genotype are shown in C. Differences in colon length (cm) by genotype are shown in D. The DSS cohort represents n = 13 WT mice (4 F 9 M), n = 13 HET (4 F 9 M), and n = 15 MUT (3 F 12 M) mice. In another cohort of 2–3-month-old TNBS mice, mice were pre-sensitized on Day 0 and injected intrarectally with TNBS on Day 7. Body weight loss assessed as % of baseline was tracked for 3 days following the injection (E). Distributions of colon length (cm) by genotype for TNBS mice are shown in F. Representative images were obtained through brightfield microscopy at 20× magnification (B) with a scale bar representing 500 microns. The TNBS cohort was composed of n = 20 WT (8 F 12 M), n = 22 HET (8 F 14 M), and n = 17 MUT (5 F 12 M) mice