TABLE 3.

Parameters for binding of Pol-derived peptides to UL42^a

Peptide	Stoichiometry	ΔH (kcal/mol)	Kd (μM)	ΔG (kcal/mol)	TΔS (kcal/mol)
A	0.89 ± 0.17	−6.2 ± 1.4	1.4 ± 0.8	−8.4 ± 1.8	−2.2 ± 1.2
E	1.06 ± 0.16	−5.7 ± 1.2	1.7 ± 0.7	−8.1 ± 1.9	−2.4 ± 1.5
G	0.81 ± 0.11	−2.2 ± 0.7	1.9 ± 0.4	−7.9 ± 0.4	−5.7 ± 0.2

^aTitrations were performed with 10-μl injections of peptide into a sample cell containing ΔC340. Heats of dilution of both peptide and protein were subtracted from the raw data prior to analysis. Nonlinear least-squares analysis of the raw data was performed with the fitting algorithm provided with the calorimeter. Values are the means of at least three determinations ± standard deviation.